Different HPLC Sampling Vial Test

Different HPLC Sampling Vial Test

Is things better if pay more price?
What’s the difference of HPLC vials from different brands

As a professional mass spectrometry testing company, the choice of experimental consumables has always been based on data. In the blank experiment, there will always be inexplicable ghost peaks, from the solvent? From the vial? Or plastic products from the extraction process? We will all check one by one with the spirit of breaking the casserole and asking to the end. In order to ensure the quality of testing, we have carried out a series of evaluations for the sample vials, just to find the most reliable one. Take a look at our evaluation results, maybe you, as a peer, are also inspired?

11 types of injection bottles and 1 type of intubation tube participating in this evaluation. Let’s see what the injection bottles look like~~~

summary
1. The material of the injection bottle is relatively uniform, the appearance is symmetrical, and the batch consistency is good.
2. The material of the sample bottle /inner tube is plastic and glass, and the price of glass is more expensive .
3. The unit price of the plastic inner tube integrated injection bottle is around 1 yuan, and the price difference is not large.
4. J plastic inner tube type D needs to be used with flat -bottom injection bottle , which is more expensive than the plastic inner tube integrated injection bottle .
5. The unit price of the glass inner tube integrated injection bottle is between 3 yuan and 7 yuan, and the price gap is large, and the price of the safety type B is the highest.
6. The price of glass injection vials (flat bottom, no inner tube) is 0.6-2 yuan, and the price of A is the highest.

Take a look at our next test results ~~ Experimental conditions
Experimental equipment: LC /MS (1290-6470) Mobile phase: A: pure water B: acetonitrile Flow rate: 0.3ml/min
Method: full scan in positive mode and negative mode Experimental steps:

Accurately measure a certain volume of pure water and
acetonitrile (the sampling volume of the integrated injection bottle with the insert tube and the insert tube is 100 μL, and the sampling volume of the injection bottle without the insert tube is 500 μL.) into each type of injection bottle , vortex for 1min, use the same brand and model cap (Agilent 5190-9024), and inject the sample on the machine. Perform full scans in positive and negative ion modes, respectively.

Injection vial + acetonitrile positive ion mode

Note: The smaller the number of peaks and the peak height, the better. The above data are for reference only, and are only responsible for the test samples.

Injection bottle + water positive ion mode

Note: The smaller the number of peaks and the peak height, the better. The above data are for reference only, and are only responsible for the test samples.

Sampling vial + acetonitrile negative ion mode

Note: The smaller the number of peaks and the peak height, the better. The

above data are for reference only, and are only responsible for the test samples.

Injection bottle + water negative ion mode

Note: The smaller the number of peaks and the peak height, the better. The

above data are for reference only, and are only responsible for the test samples.

Form arrangement:

summary
chemical performance of the Spectrum J injection vial and the A injection vial are better, but the latter has poor repeatability. The B model also performed very well, but the price was on the high side. It seems that expensive things make a little sense, but I always feel that the brand premium is a little more. I wonder if you feel the same way~~

Summarize
various brands of injection bottles . These signals will cause unknown effects on our target detection, but it is difficult to find an absolutely “clean” injection in the market. bottle. sampling vials and inserts participating in the evaluation , the J type sampling vial is worth choosing, but it is not suitable for micro samples (about <500μL). The B model is also good. The integrated design of the inner intubation tube has a wide range of applications, but it is expensive.